Cheon Lab

Main Links

Logo and Main Menu

Full Menu


Yiseul Ryu†, Zongwen Jin†, Joong-Jae Lee, Seung-Hyun Noh, Tae-Hyun Shin, Seong-Min Jo, Joonsung Choi, Hyun Wook Park, Jinwoo Cheon, and Hak-Sung Kim* († equally contributed)
Size-Controlled Construction of Magnetic Nanoparticle Clusters Using DNA-Binding Zinc Finger Protein
Angew. Chem. Int. Ed., 54 (3), 923-926
Date: Nov 25, 2014

Nanoparticle clusters (NPCs) have attracted significant interest owing to their unique characteristics arising from their collective individual properties. Nonetheless, the construction of NPCs in a structurally well‐defined and size‐controllable manner remains a challenge. Here we demonstrate a strategy to construct size‐controlled NPCs using the DNA‐binding zinc finger (ZnF) protein. Biotinylated ZnF was conjugated to DNA templates with different lengths, followed by incubation with neutravidin‐conjugated nanoparticles. The sequence specificity of ZnF and programmable DNA templates enabled a size‐controlled construction of NPCs, resulting in a homogeneous size distribution. We demonstrated the utility of magnetic NPCs by showing a three‐fold increase in the spin–spin relaxivity in MRI compared with Feridex. Furthermore, folate‐conjugated magnetic NPCs exhibited a specific targeting ability for HeLa cells. The present approach can be applicable to other nanoparticles, finding wide applications in many areas such as disease diagnosis, imaging, and delivery of drugs and genes.

Copyright and Address

  • ADDRESS Department of Chemistry. 50 Yonsei-ro, Seodaemun-gu, Seoul 03722
  • TEL +82-2-2123-4769
  • FAX +82-2-2123-4640
  • © Cheon Group | Yonsei University. All Right Reserved

Display Page Loading Image