Cheon Lab

Main Links

Logo and Main Menu

Full Menu

Research

Kisoo Park, Hoyoung Kim, Soojin Kim, Kyungheon Lee, Sohyeon Park, Jun Song, Changwook Min, Farhana Khanam, Rasheduzzaman Rashu, Taufiqur Rahman Bhuiyan, Edward T. Ryan, Firdausi Qadri, Ralph Weissleder, Jinwoo Cheon*, Richelle C. Charles*, and Hakho Lee*
Nanomagnetic System for Rapid Diagnosis of Acute Infection
ACS Nano, 11 (11), 11425-11432
Date: Nov 9, 2017

Pathogen-activated antibody-secreting cells (ASCs) produce and secrete antigen-specific antibodies. ASCs are detectable in the peripheral blood as early as 3 days after antigen exposure, which makes ASCs a potential biomarker for early disease detection. Here, we present a magnetic capture and detection (MCD) assay for sensitive, on-site detection of ASCs. In this approach, ASCs are enriched through magnetic capture, and secreted antibodies are magnetically detected by a miniaturized nuclear magnetic resonance (μNMR) system. This approach is based entirely on magnetics, which supports high contrast against biological background and simplifies assay procedures. We advanced the MCD system by (i) synthesizing magnetic nanoparticles with high magnetic moments for both cell capture and antibody detection, (ii) developing a miniaturized magnetic device for high-yield cell capture, and (iii) optimizing the μNMR assay for antibody detection. Antibody responses targeting hemolysin E (HlyE) can accurately identify individuals with acute enteric fever. As a proof-of-concept, we applied MCD to detect antibodies produced by HlyE-specific hybridoma cells. The MCD achieved high sensitivity in detecting antibodies secreted from as few as 5 hybridoma cells (50 cells/mL). Importantly, the assay could be performed with whole blood with minimal sample processing.

Copyright and Address

  • ADDRESS Department of Chemistry. 50 Yonsei-ro, Seodaemun-gu, Seoul 03722
  • TEL +82-2-2123-4769
  • FAX +82-2-2123-4640
  • EMAIL lee7626@yonsei.ac.kr
  • © Cheon Group | Yonsei University. All Right Reserved

Display Page Loading Image

Top